Albion manager Martin Hinshelwood has urged his players to turn Withdean into a fortress again.
Rock-solid home form was responsible for the Seagulls' successive titles, but they are seeking their first Withdean win in Division One in the basement scrap against Grimsby.
Hinshelwood, boosted by the prospect of having Bobby Zamora, Graham Barrett and Michel Kuipers back, said: "It's a big game for us.
"We are back at home after a couple away and we need to start getting results again at Withdean. It has been a bit of a fortress for the last couple of years.
"We know it's going to be difficult. They are below us in the League and they are not going to make it easy for us. It's a game where we need to be patient more than anything over the 90 minutes."
Zamora and Barrett are poised to partner each other up front. Zamora has trained all week after a nine-game absence with knee ligament damage, while Barrett makes his home debut a month after signing on loan from Arsenal.
"You do miss people with that something extra," Hinshelwood said. "Any manager in the country would like to have someone like Bobby who has scored 60 goals in two years.
"Graham is looking forward to it and I am sure people will see that he is one for the future at Arsenal.
"It's going to take a while for them to bed in again. It's a bit different for Michel as a goalkeeper, but with the two up front it will take a bit of time for them to get used to each other.
"It's like having two new players in the side and they are quality players."
Gary Hart, ready for a return to the right flank tomorrow, said: "I had to play up on my own against Ipswich. I tried to do a job, but I'm not a prolific goalscorer like Bobby. We need someone up there who can put it in the back of the net.
"I've only seen Graham in training, but I'm told he is a really good player. Anyone who is at Arsenal must be reasonable!"
Young strikers Shaun Wilkinson (tonsilitis) and Daniel Marney (hamstring) resumed training yesterday, but John Piercy's home debut has been put on hold by a leg injury sustained in the first half of the Worthington Cup exit at Ipswich.
Piercy joins Paul Kitson (back), Nathan Jones (knee), Robbie Pethick (foot) and Geoff Pitcher (heel) on the casualty list.
Albion are next-to-bottom with a five-point gap to bridge after eight straight League defeats, but Hinshelwood added: "You always feel as though a result will turn it around. If we can get a result under our belt then I think we will start flying."
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