Stars will lead the way during a one-mile run for the charity Sports Relief.
Sussex-born Harry Enfield, presenter Nick Knowles, former S Club singer Rachel Stevens, Tess Daly, Gaby Logan, Gary Lineker, hurdler Colin Jackson and Olympic gold medallist Sir Steve Redgrave are all taking part in the event on July 10.
Organisers have not yet confirmed which celebrities will be hosting Brighton's mile - one of hundreds across the UK.
However, at least one high-profile star will lead hundreds of entrants helping to raise money for the disadvantaged in the UK and abroad.
This year's theme is Go The Extra Mile and organisers hope thousands will join in.
The Brighton Mile will take place in Preston Park.
Each event for Sports Relief, the sporting arm of Comic Relief, will be led by a celebrity host. Participants are being encouraged to complete a mile in whichever way they chose.
A well-known BBC presenter is also expected to compere at the event.
Other venues for running events around the UK include London, Edinburgh, Belfast, Cardiff, and Manchester.
Five-times Olympic champion Sir Steve Redgrave said: "If you do one thing this summer, make sure it is the Fitness First Sport Relief Mile.
"I got involved with Sport Relief on its debut two years ago when it raised £14.4 million - money that is already helping people who are forced to live really tough lives here in the UK and in some of the poorest countries around the world.
"This time around, thousands and thousands of people will be taking part in the biggest mile event in history to raise even more cash."
People will have the choice of joining the the Time Trial Mile - for those who want to set themselves a target to beat on the day - or the more relaxed Freestyle Mile.
A spokesman said: "We are calling on the nation to run, walk, hop, wear something silly - do whatever they want."
Rachel Stevens is also recording a single, Some Girls, to raise money for the charity.
Money raised from the last Sport Relief event has been used to support a number of good causes, including street children in India and a project to promote racial harmony in Camden, London.
The 2002 event attracted stars such as David Beckham, Nelson Mandela, Muhammad Ali and Michael Owen.
An evening of entertainment followed on BBC One, most memorably involving a celebrity boxing match between comedians Les Dennis and Bob Mortimer.
This year's event will be launched live on Grandstand at 12.50pm on July 10.
For more information, visit www.sportrelief.com
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