Twenty new jobs will be created when a supermarket starts 24-hour trading next month.
Asda at Brighton Marina is to open round the clock from Monday to Saturday from September 11.
The store will be recruiting shift workers during the next month.
It is one of 86 across the country to extend its opening hours.
Chief operating officer Paul Mason said: "These days people need the flexibility of service.
"Whether they work odd hours and need to do the family shop late, or they have to buy something at the last minute, visitors to the marina will find this service extremely useful."
Asda's Hollingbury store started opening 24 hours a day two years ago.
Nationally, the company will create around 800 jobs through the change.
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