Paul Stokes added to the woes of Lewes after breaking an ankle in last week's win over Leyton Pennant.
Stokes joins a long injury list at the Dripping Pan which also includes Stuart Hack, Andy Johnson, Peter Brackley and Sam Francis.
With a limited squad, Rooks boss Jimmy Quinn may be forced to look for replacements, although he admits it will be tough with little money to play with.
Influential midfielder Stokes has been ruled out for the season after having pins inserted in the ankle. He was released from hospital on Monday but has had a bad week after suffering an adverse reaction to medication he was given.
Quinn said: "Paul has been a good player for us. A bit like Hacky (Stuart Hack), he will be badly missed.
"Leyton Pennant have not been a good side for us. It was the same team against whom we lost Ryan Hackett earlier in the season."
Ironically, Hackett made his return to the squad with a place on the bench in last Saturday's 2-0 victory. It was his first taste of action since September.
Paul Thomsett was due to return from a ban for today's trip to Wembley but Justin Harris has only just started a four match suspension.
Chichester City United boss Adie Girdler is looking to blood some youngsters in the new year. Girdler said: "I intend to give some of the kids a go." Matt Smith and Matt Osborne are two of the youngsters set to make an impact.
Wick chief Carl Stabler has admitted his New Year's resolution is "to ensure we don't get relegated." Stabler has a fully fit squad at his disposal as he bids to rid the club of the spectre of relegation. Ben Milford and Gareth Green return to the squad at Crabtree Park.
Littlehampton manager Andy Taylor's squad is almost at full strength with Derren Woods and Barry Pidgeon both back in first team action after injuries. Striker Woods and Pidgeon both came on as subs in last Saturday's win over Eastbourne United.
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