Commercial growing of GM crops is attracting more resistance.
A debate organised by Brighton and Hove City Council's sustainability commission ended in unanimous opposition to the controversial move.
Many people raised fears about the safety of growing GM crops, their impact on the environment, consumer choice and global markets.
They said crops were being introduced too quickly with too little research.
Commission convenor Joyce Edmond Smith said: "It shows people simply do not want GM crops."
The debate was part of the Government's national GM Nation consultation.
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