Uckfield did a Haywards Heath after winning promotion. They scored in the first minute of their home game, took a big lead but lost it at the death.
Three tries in the last ten minutes did the damage as Aylesford pinched a 32-26 verdict in London Four South East.
Nick Ridley's super pick-up set up Lee Evans to score after 30 seconds, with Ed Gannon's conversion making it a dream start.
Aylesford's large pack were always a major factor but Uckfield's pace outside looked to be winning the day as skipper Phil Rowland scored a hat-trick of tries.
Two first-half scores, both goaled by Gannon, made it 21-10 at the break.
He pushed the lead back out to 26-15 after being sent to the line by Nick Crane.
East Grinstead had a dreadful day at Folkestone. They turned up late due to heavy traffic, then saw their young side crash 36-5.
Kevin Pike scored the only try for Gs.
Eastbourne are similarly short of experience. The average age of their pack was only 22 as they lost 31-15 at home to Old Dunstonian.
Poor tackling did not help, with stand-in skipper Danny Craven and overseas player Thomas Kohi the only exceptions to that rule.
Craven added a touchline conversion to Paul Newell's try and kicked a penalty as Eastbourne trailed 20-10 at the break.
Mark Ashworth touched down in the second period.
Crawley went down 17-6 at Bromley and felt two Andy Evans penalties were scant reward for the possession and territorial advantage they enjoyed.
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